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Bovine Prokineticin Receptor 1 (PKR1) ELISA Kit

SKU:
BODL00140
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8SPN2
Sensitivity:
0.23ng/mL
Range:
0.937-60ng/mL
ELISA Type:
Sandwich
Synonyms:
PROKR1,PKR-1,GPR73,GPR-73a,ZAQ,PK-R1,G Protein-Coupled Receptor 73,G-protein coupled receptor ZAQ
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Bovine Prokineticin Receptor 1 (PKR1) ELISA Kit

The Bovine Prokineticin Receptor 1 (PKR1) ELISA Kit is a reliable tool for the precise measurement of PKR1 levels in bovine serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Prokineticin receptor 1 is a key component in regulating various biological processes, including reproductive functions, circadian rhythms, and pain perception.

It plays a crucial role in mediating the effects of prokineticins, which are involved in a wide range of physiological and pathological conditions.By accurately quantifying PKR1 levels, researchers can gain valuable insights into the role of prokineticin signaling in different biological processes and diseases. This ELISA kit provides a valuable tool for studying the functions of PKR1 and developing potential therapeutic strategies targeting this receptor.

Product Name:Bovine Prokineticin Receptor 1 (PKR1) ELISA Kit
Product Code:BODL00140
Size:96 Assays
Target:PKR1
Synonyms:PROKR1, PKR-1, GPR73, GPR-73a, ZAQ, PK-R1, G Protein-Coupled Receptor 73, G-protein coupled receptor ZAQ
Detection Method:Sandwich
Range:0.937-60ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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