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Bovine Polymeric Immunoglobulin Receptor (PIGR) ELISA Kit

SKU:
BODL00138
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P81265
Sensitivity:
0.238ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
SC,Secretory Component,Hepatocellular carcinoma-associated protein TB6
Reactivity:
Bovine
Sample Type:
Serum, Plasma And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Bovine Polymeric Immunoglobulin Receptor (PIGR) ELISA Kit

The Bovine Polymeric Immunoglobulin Receptor (PIgR) ELISA Kit is specifically designed for the quantitative measurement of PIgR levels in bovine serum, plasma, and tissue homogenates. This ELISA kit offers exceptional sensitivity and specificity, providing researchers with reliable and consistent results for their studies.PIgR is a key component of the mucosal immune system, playing a vital role in the transportation of immunoglobulins across mucosal surfaces. Dysregulation of PIgR expression has been linked to various mucosal diseases and immune disorders, making it a valuable biomarker for research in these areas.

By utilizing the Bovine PIgR ELISA Kit, researchers can gain insight into the role of PIgR in mucosal immunity and inflammatory responses, facilitating advancements in the understanding and treatment of related diseases. This kit is an essential tool for any study involving bovine PIgR and its associated biological processes.

Product Name:Bovine Polymeric Immunoglobulin Receptor (PIGR) ELISA Kit
Product Code:BODL00138
Size:96 Assays
Target:PIGR
Synonyms:SC, Secretory Component, Hepatocellular carcinoma-associated protein TB6
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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