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Bovine Placenta Growth Factor (PLGF) ELISA Kit

SKU:
BODL00141
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9XS47
Sensitivity:
6.8pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
PlGF2,PGF,PGFL,Placental Growth Factor-Like,Vascular Endothelial Growth Factor-Related Protein
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Bovine Placenta Growth Factor (PLGF) ELISA Kit

The Bovine Placenta Growth Factor (PLGF) ELISA Kit is a reliable tool for the accurate measurement of PLGF levels in bovine serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, allowing for precise and reproducible results, making it well-suited for various research applications.PLGF is a key growth factor involved in the regulation of vascular development and angiogenesis. It plays a crucial role in promoting blood vessel growth and maintenance, making it a valuable biomarker for studying conditions such as pregnancy complications, cardiovascular diseases, and cancer.

The Bovine PLGF ELISA Kit provides researchers with a valuable tool for investigating the role of PLGF in different physiological and pathological processes, ultimately aiding in the development of potential therapeutic interventions.

Product Name:Bovine Placenta Growth Factor (PLGF) ELISA Kit
Product Code:BODL00141
Size:96 Assays
Target:PLGF
Synonyms:PlGF2, PGF, PGFL, Placental Growth Factor-Like, Vascular Endothelial Growth Factor-Related Protein
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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