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Bovine Ornithine Carbamoyl Transferase (OCT) ELISA Kit

SKU:
BODL00130
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9N1U7
Sensitivity:
0.135ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
OCTD,OTCase,Ornithine Transcarbamylase,Ornithine Carbamoyltransferase
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Bovine Ornithine Carbamoyl Transferase (OCT) ELISA Kit

The Bovine Ornithine Carbamoyl Transferase (OCT) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of OCT levels in bovine serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.OCT is an important enzyme involved in the urea cycle, playing a key role in the metabolism of nitrogen compounds in bovine cells. Dysregulation of OCT activity has been linked to various metabolic disorders and liver diseases in cattle, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.

Whether you are conducting basic research or clinical studies in veterinary medicine, the Bovine OCT ELISA Kit provides a powerful tool for quantifying OCT levels and advancing your understanding of bovine metabolic pathways. Trust Assay Genie for reliable results and unparalleled quality in your research endeavors.

Product Name:Bovine Ornithine Carbamoyl Transferase (OCT) ELISA Kit
Product Code:BODL00130
Size:96 Assays
Target:OCT
Synonyms:OCTD, OTCase, Ornithine Transcarbamylase, Ornithine Carbamoyltransferase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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