null

Bovine Mucin 1 (MUC1) ELISA Kit

SKU:
BODL00126
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8WML4
Sensitivity:
0.098ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD227,EMA,H23AG,KL-6,PEM,PEMT,PUM,CA15-3,Cancer antigen 15-3,Carcinoma-associated mucin,Episialin,Peanut-reactive urinary mucin,Polymorphic epithelial mucin
Reactivity:
Bovine
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Bovine Mucin 1 (MUC1) ELISA Kit

The Bovine Mucin 1 (MUC1) ELISA Kit is specially designed for the accurate and reliable detection of MUC1 levels in bovine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides researchers with precise and reproducible results, making it an invaluable tool for various research applications.MUC1 is a transmembrane glycoprotein that plays a key role in cell adhesion, signaling, and immune response modulation.

Aberrant expression of MUC1 has been linked to various diseases, including cancer and inflammatory conditions, making it a valuable biomarker for studying the pathogenesis of these disorders and developing potential therapeutic strategies.Overall, the Bovine MUC1 ELISA Kit offers researchers a reliable and efficient method for quantifying MUC1 levels in bovine samples, advancing our understanding of the role of MUC1 in health and disease.

Product Name:Bovine Mucin 1 (MUC1) ELISA Kit
Product Code:BODL00126
Size:96 Assays
Target:MUC1
Synonyms:CD227, EMA, H23AG, KL-6, PEM, PEMT, PUM, CA15-3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, Peanut-reactive urinary mucin, Polymorphic epithelial mucin
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products