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Bovine Matrix Metalloproteinase 7 (MMP7) ELISA Kit

SKU:
BODL00123
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q148N3
Sensitivity:
0.089ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
MPSL1,MAT,PUMP-1,Matrilysin Uterine,Matrilysin,Matrin,Pump-1 Protease,Uterine Metalloproteinase
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Bovine Matrix Metalloproteinase 7 (MMP7) ELISA Kit

The Bovine Matrix Metalloproteinase-7 (MMP7) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of MMP7 levels in bovine serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it the perfect tool for a wide range of research applications.MMP7 is a key enzyme involved in extracellular matrix remodeling, playing a crucial role in tissue regeneration and repair. Dysregulation of MMP7 has been linked to various diseases, including cancer, inflammatory disorders, and fibrotic conditions.

By measuring MMP7 levels, researchers can gain valuable insights into disease pathogenesis, progression, and potential therapeutic interventions.This MMP7 ELISA kit is easy to use and comes with all the necessary components for accurate and efficient detection. With its high sensitivity and specificity, researchers can trust in the results obtained using this kit for their research needs.

Product Name:Bovine Matrix Metalloproteinase 7 (MMP7) ELISA Kit
Product Code:BODL00123
Size:96 Assays
Target:MMP7
Synonyms:MPSL1, MAT, PUMP-1, Matrilysin Uterine, Matrilysin, Matrin, Pump-1 Protease, Uterine Metalloproteinase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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