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Bovine Matrix Metalloproteinase 23B (MMP23B) ELISA Kit

SKU:
BODL00121
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q2TBM7
Sensitivity:
0.073ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MMP22,MMP23-B,MIFR,MIFR-1,Matrix Metalloproteinase 22,Femalysin,Matrix Metalloproteinase In The Female Reproductive Tract
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Bovine Matrix Metalloproteinase 23B (MMP23B) ELISA Kit

The Bovine Matrix Metalloproteinase-23B (MMP23B) ELISA Kit is a powerful tool for accurate measurement of MMP23B levels in bovine samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.MMP23B is a key enzyme involved in extracellular matrix remodeling, playing a critical role in processes such as tissue development, wound healing, and inflammation. Dysregulation of MMP23B has been linked to various diseases, including cancer, arthritis, and pulmonary fibrosis, highlighting its importance as a potential therapeutic target and biomarker for disease progression.

Overall, the Bovine MMP23B ELISA Kit provides researchers with a valuable tool to study the function and regulation of MMP23B in various physiological and pathological conditions, ultimately leading to a better understanding of disease mechanisms and the development of targeted therapies.

Product Name:Bovine Matrix Metalloproteinase 23B (MMP23B) ELISA Kit
Product Code:BODL00121
Size:96 Assays
Target:MMP23B
Synonyms:MMP22, MMP23-B, MIFR, MIFR-1, Matrix Metalloproteinase 22, Femalysin, Matrix Metalloproteinase In The Female Reproductive Tract
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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