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Bovine Matrix Metalloproteinase 13 (MMP13) ELISA Kit

SKU:
BODL00119
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O77656
Sensitivity:
33pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
CLG3,Collagenase 3
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Bovine Matrix Metalloproteinase 13 (MMP13) ELISA Kit

The Bovine Matrix Metalloproteinase-13 (MMP13) ELISA Kit is specifically designed for the accurate and precise measurement of MMP13 levels in bovine samples. This kit offers high sensitivity and specificity, allowing for reliable and reproducible results in a variety of research applications.MMP13 is a key enzyme involved in the breakdown of extracellular matrix components, playing a crucial role in tissue remodeling and repair processes. Dysregulation of MMP13 has been implicated in various pathological conditions, including arthritis, cancer, and cardiovascular diseases, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.

With easy-to-follow protocols and quick assay times, the Bovine MMP13 ELISA Kit is an essential tool for researchers interested in studying the role of MMP13 in physiological and pathological processes in bovine samples.

Product Name:Bovine Matrix Metalloproteinase 13 (MMP13) ELISA Kit
Product Code:BODL00119
Size:96 Assays
Target:MMP13
Synonyms:CLG3, Collagenase 3
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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