Bovine Interleukin Array (11 targets) (SARB0004)
- SKU:
- SARB0004
- Product Type:
- Protein Array
- Analytes:
- IL-1 alpha (IL-1 F1)
- IL-1 beta (IL-1 F2)
- IL-36 Ra (IL-1 F5)
- IL-10
- IL-13
- IL-15
- IL-17A
- IL-18
- IL-2
- IL-21
- IL-4
- Reactivity:
- Bovine
- Applications:
- Multiplex Array
Description
Bovine Interleukin Array (11 targets) (SARB0004)
The Bovine Interleukin Array kit (SARB0004) from Assay Genie is a powerful tool for researchers studying bovine immune responses and inflammatory conditions. This array kit allows for the simultaneous detection of 11 different interleukins, providing valuable insights into cytokine signaling and immune system modulation in bovine samples.Each antibody in the kit is rigorously validated for specificity and sensitivity, ensuring accurate and reliable results. The array can be used with a variety of sample types, including serum, plasma, and cell culture supernatants, making it versatile for different experimental setups.
With the ability to detect multiple interleukins in a single assay, researchers can quickly assess the cytokine profile of their samples and uncover potential biomarkers or therapeutic targets. The Bovine Interleukin Array kit is a valuable resource for studies in veterinary medicine, immunology, and livestock health.
| Product Sku: | SARB0004 |
| Size: | 2, 4, or 8 |
| Species Detected: | Bovine |
| Number of Targets Detected: | 11 |
| Gene Symbols: | IL10, IL13, IL15, IL17A, IL18, IL1A, IL1B, IL2, IL21, IL36RN|IL4 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-36 Ra (IL-1 F5)
| IL-10
| IL-13
|
IL-15
| IL-17A
| IL-18
| IL-2
| IL-21
|
IL-4
| ||||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Bovine Interleukin Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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