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Bovine Interleukin 10 (IL-10) ELISA Kit

SKU:
BODL00086
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P43480
Sensitivity:
5.7pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CSIF,IL10A,TGIF,Cytokine Synthesis Inhibitory Factor
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Bovine Interleukin 10 (IL-10) ELISA Kit

The Bovine Interleukin-10 (IL-10) ELISA Kit is a powerful tool for measuring levels of IL-10 in bovine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it perfect for a variety of research purposes.IL-10 is a key immunoregulatory cytokine that plays a crucial role in modulating the immune response and inflammation.

Its dysregulation is implicated in various diseases, including autoimmune disorders, infectious diseases, and cancer. By using the Bovine IL-10 ELISA Kit, researchers can gain valuable insights into the role of IL-10 in bovine health and disease. This kit is essential for studying immune responses, evaluating therapeutic interventions, and advancing our understanding of bovine immunology.

Product Name:Bovine Interleukin 10 (IL-10) ELISA Kit
Product Code:BODL00086
Size:96 Assays
Target:IL10
Synonyms:CSIF, IL10A, TGIF, Cytokine Synthesis Inhibitory Factor
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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