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Bovine Interferon Alpha (IFNa) ELISA Kit

SKU:
BODL00074
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q04790
Sensitivity:
5.9pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
IFNA1,IFL,LeIF D,IFN,IFN-Alpha,IFNA13,IFN-A,IFNAP22,IFN-Alpha 1b,Interferon Alpha 1b,Interferon, Leukocytic,IFN, Leukocyte,Interferon alpha-D
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Bovine Interferon Alpha (IFNa) ELISA Kit

The Bovine Interferon Alpha (IFN-Alpha) ELISA Kit is a powerful tool for detecting and quantifying levels of IFN-Alpha in bovine serum, plasma, and cell culture supernatants. This kit is specifically designed to provide highly accurate and reliable results, with exceptional sensitivity and specificity.Interferon Alpha is a key player in the immune response, playing a crucial role in host defense against viral infections and other pathogens.

By accurately measuring IFN-Alpha levels, researchers can gain valuable insights into immune function and disease progression in bovine species.Whether studying infectious diseases, immune responses, or vaccine development in bovines, the Bovine Interferon Alpha ELISA Kit is an essential tool for researchers looking to advance their understanding of immune function and develop new therapeutic strategies.

Product Name:Bovine Interferon Alpha (IFNa) ELISA Kit
Product Code:BODL00074
Size:96 Assays
Target:IFNa
Synonyms:IFNA1, IFL, LeIF D, IFN, IFN-Alpha, IFNA13, IFN-A, IFNAP22, IFN-Alpha 1b, Interferon Alpha 1b, Interferon, Leukocytic, IFN, Leukocyte, Interferon alpha-D
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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