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Bovine Insulin Like Growth Factor 1 (IGF1) ELISA Kit

SKU:
BODL00078
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
F2X2F1
Sensitivity:
1.23ng/mL
Range:
3.12-200ng/mL
ELISA Type:
Sandwich
Synonyms:
IGFI,IGF1A,IBP1,MGF,Somatomedin C,Mechano Growth Gactor
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Bovine Insulin Like Growth Factor 1 (IGF1) ELISA Kit

The Bovine Insulin-Like Growth Factor 1 (IGF1) ELISA Kit is a powerful tool for accurately measuring levels of IGF1 in bovine serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.IGF1 is a vital growth factor that plays a key role in regulating growth, development, and metabolism in cattle. It is involved in various biological processes, including cell proliferation, differentiation, and survival.

The accurate measurement of IGF1 levels is essential for studying growth disorders, metabolic diseases, and the effects of nutrition on livestock health.Whether you are conducting basic research or seeking to improve animal health and productivity, the Bovine IGF1 ELISA Kit provides a reliable and efficient solution for measuring IGF1 levels in bovine samples. Unlock new insights into bovine growth and development with this advanced and user-friendly ELISA kit.

Product Name:Bovine Insulin Like Growth Factor 1 (IGF1) ELISA Kit
Product Code:BODL00078
Size:96 Assays
Target:IGF1
Synonyms:IGFI, IGF1A, IBP1, MGF, Somatomedin C, Mechano Growth Gactor
Detection Method:Sandwich
Range:3.12-200ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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