Bovine GM-CSF DIY ELISA Kit
- SKU:
- KES0203
- Product Type:
- ELISA Kit
- Reactivity:
- Bovine
- Applications:
- ELISA
- ELISA Type:
- DIY ELISA
- Size:
- 10 Plates
- Synonyms:
- CSF2, GMCSF
Description
Bovine GM-CSF DIY ELISA Kit
The Bovine GM-CSF ELISA Kit is specifically designed for the precise measurement of GM-CSF levels in bovine serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.GM-CSF, or granulocyte-macrophage colony-stimulating factor, is a vital cytokine that plays a pivotal role in the regulation of immune responses and inflammation. It is involved in the production and differentiation of various white blood cells, making it a critical component in the body's defense against infections and diseases.
The measurement of GM-CSF levels is essential in studying inflammatory processes, autoimmune disorders, and immunodeficiency conditions, providing valuable insights for potential therapeutic interventions.Overall, the Bovine GM-CSF ELISA Kit is an indispensable tool for researchers looking to delve deeper into the intricate mechanisms of immune regulation and inflammation in bovine species. With its advanced technology and accuracy, this kit offers a reliable solution for exploring the multifaceted roles of GM-CSF in various physiological and pathological conditions.
| Product Name: | Bovine GM-CSF DIY ELISA |
| Product Code: | KES0203 |
| Species: | Bovine |
| Target: | GM-CSF |
| Synonyms: | CSF2, GMCSF |
| Application: | ELISA |
| ELISA Type: | DIY ELISA Kit |
| Size: | 10 Plates |
| Shipping: | Room temperature |
| Storage: | Stable for up to twelve months from date of receipt at 2-8°C. |
| Description | Usage | Quantity |
| Anti-Bovine GM-CSF Polyclonal Antibody | Capture Antibody | 100 µg |
| Biotinylated Anti-Bovine GM-CSF Polyclonal Antibody | Detection Antibody | 50 µg |
| Bovine GM-CSF Recombinant Protein | Standard | 5 µg |
| Reagent | Suggested Formulation |
| DPBS: | 0.008M sodium phosphate, 0.002M potassium phosphate, 0.14M sodium chloride, 0.01M potassium chloride, pH 7.4 |
| 96-well ELISA Plate: | Clear, flat-bottom, high-binding 96-well plate, 8-wells per strip, 350 µL per well (ELISA Plates: KESAP003) |
| Standard and Sample Diluent: | The optimal Standard and Sample Diluent will need to be determined for each sample type to obtain optimal recovery and linearity. The appropriate Standard and Sample Diluent will mimic the sample's response to a known quantity of protein standard and will provide linear results when diluted. Often a 1:4 dilution of the sample in Reagent Diluent will provide acceptable recovery and linearity. |
| Reagent Diluent and Blocking Buffer: | 4% BSA in DPBS, 0.2 µm filtered |
| Wash Buffer: | 0.05% Tween®-20 in DPBS |
| Streptavidin-HRP: | Enzymatic reagent to react with biotinylated detection antibody (Streptavidin-HRP: KESAP002) |
| Substrate: | 3,3',5,5'-tetramethylbenzidine (TMB) Substrate (ELISA Accessory Pack: KESAP001) |
| Stop Solution: | 0.18 M Sulfuric Acid (ELISA Accessory Pack: KESAP001) |
| Plate Sealer: | Adhesive film to prevent evaporation |
*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1. | Prepare Capture Antibody in DPBS at desired working concentration. |
| 2. | Add 100 µL of Capture Antibody Working Solution to appropriate wells. |
| 3. | Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 12-24 hours. |
| 4. | Empty Capture Antibody Working Solution from plate. Blot plate onto paper towels or other absorbent material. |
| 5. | Add 100 µL of Blocking Buffer to appropriate wells. |
| 6. | Cover plate with Plate Sealer and incubate at room temperature for 1-3 hours. |
| 7. | Empty Blocking Buffer from plate. Blot plate onto paper towels or other absorbent material. |
| 8. | Prepare Standard and sample as desired with Standard and Sample Diluent. |
| 9. | Add 100 µL of Standard or sample to appropriate wells. Note: Run each Standard or sample in duplicate. |
| 10. | Cover plate with Plate Sealer and incubate at room temperature for 1 hour. |
| 11. | Wash plate FOUR times with Wash Buffer. Note: Gently squeeze the long sides of plate frame before washing to ensure all strips remain securely in the frame. Empty plate contents. Use a squirt wash bottle to vigorously fill each well completely with 1X Wash Buffer, then empty plate contents. Repeat procedure three additional times for a total of FOUR washes. Blot plate onto paper towels or other absorbent material. |
| 12. | Prepare Detection Antibody in Reagent Diluent at desired working concentration. |
| 13. | Add 100 µL of Detection Antibody Working Solution to each well. |
| 14. | Cover plate with Plate Sealer and incubate at room temperature for 1 hour. |
| 15. | Wash plate FOUR times with Wash Buffer as described in step 11. |
| 16. | Prepare Streptavidin-HRP in Reagent Diluent at desired working concentration. |
| 17. | Add 100 µL of Streptavidin-HRP Working Solution to each well. |
| 18. | Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. |
| 19. | Wash plate FOUR times with Wash Buffer as described in step 11. |
| 20. | Add 100 µL of TMB Substrate Solution to each well. |
| 21. | Develop the plate in the dark at room temperature for 30 minutes or as desired. Note: Do NOT cover plate with Plate Sealer. |
| 22. | Stop reaction by adding 100 µL of Stop Solution to each well. |
| 23. | Measure absorbance on a plate reader at 450 nm. |
The Bovine GM-CSF DIY ELISA kit from Assay Genie can assay for GM-CSF in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues. Bovine GM-CSF DIY ELISA Kit from Assay Genie allows researchers to develop their own ELISA plates for GM-CSF using our unique combination of capture and detection antibodies.
Each Bovine GM-CSF DIY ELISA Kit contains capture antibody, standard, and detection antibody for development of an GM-CSF ELISA. GM-CSF antibodies in this kit have been determined to function in an ELISA with the GM-CSF standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined and require optimisation for the development of this kit. A working knowledge of ELISA is strongly recommended.
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