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Bovine Galectin 9 (GAL9) ELISA Kit

SKU:
BODL00059
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q3MHZ8
Sensitivity:
7.8pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
LGALS9,Ecalectin,Lectin,Galactoside-Binding Soluble 9,Tumor antigen HOM-HD-21
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Bovine Galectin 9 (GAL9) ELISA Kit

The Bovine Galectin-9 (Gal9) ELISA Kit is a reliable and accurate tool for the detection of Galectin-9 levels in bovine serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Galectin-9 is a protein that plays a crucial role in the immune response, regulating T cell function and promoting apoptosis in certain cell types.

Studies have shown its involvement in inflammatory diseases, cancer progression, and autoimmune disorders, making it a valuable biomarker for investigating these conditions and potential therapeutic interventions.With the Bovine Galectin-9 ELISA Kit, researchers can confidently analyze Galectin-9 levels in bovine samples, contributing to a better understanding of its role in various biological processes and disease states.

Product Name:Bovine Galectin 9 (GAL9) ELISA Kit
Product Code:BODL00059
Size:96 Assays
Target:GAL9
Synonyms:LGALS9, Ecalectin, Lectin,Galactoside-Binding Soluble 9, Tumor antigen HOM-HD-21
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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