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Bovine Galectin 8 (GAL8) ELISA Kit

SKU:
BODL00058
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q2YDD7
Sensitivity:
0.074ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
LGALS8,PCTA1,Po66-CBP,Prostate Carcinoma Tumor Antigen 1,Lectin,Galactoside-Binding Soluble 8,Po66 carbohydrate-binding protein,Prostate carcinoma tumor antigen 1
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Bovine Galectin 8 (GAL8) ELISA Kit

The Bovine Galectin-8 (Gal8) ELISA Kit is a reliable tool for the quantitative detection of Gal8 levels in bovine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it well-suited for various research applications.Gal8 is a protein known for its involvement in immune responses, cell adhesion, and signaling pathways. It plays a vital role in regulating inflammatory processes and modulating the immune system.

Studying Gal8 levels can provide valuable insights into immune-related diseases, such as autoimmune disorders and cancer, as well as potential therapeutic targets.This ELISA kit offers researchers a powerful tool for investigating the role of Gal8 in different biological processes and disease pathways, ultimately contributing to a better understanding of immune regulation and the development of novel treatments.

Product Name:Bovine Galectin 8 (GAL8) ELISA Kit
Product Code:BODL00058
Size:96 Assays
Target:GAL8
Synonyms:LGALS8, PCTA1, Po66-CBP, Prostate Carcinoma Tumor Antigen 1, Lectin,Galactoside-Binding Soluble 8, Po66 carbohydrate-binding protein, Prostate carcinoma tumor antigen 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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