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Bovine Galectin 4 (GAL4) ELISA Kit

SKU:
BODL00057
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q3T0D6
Sensitivity:
0.112ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
LGALS4,Lactose-Binding Lectin 4,L-36 Lactose-Binding Protein,L36LBP,Lectin,Galactoside-Binding Soluble 4
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Bovine Galectin 4 (GAL4) ELISA Kit

The Bovine Galectin-4 (Gal4) ELISA Kit is a powerful tool for quantitative detection of Gal4 levels in bovine serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.Gal4 is a key protein involved in cellular adhesion and signaling pathways, playing a critical role in various physiological and pathological processes.

Its dysregulation has been linked to diseases such as cancer, inflammation, and immune disorders, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.Overall, the Bovine Galectin-4 ELISA Kit provides researchers with a reliable and efficient method for studying Gal4's functions and exploring its implications in different biological contexts.

Product Name:Bovine Galectin 4 (GAL4) ELISA Kit
Product Code:BODL00057
Size:96 Assays
Target:GAL4
Synonyms:LGALS4, Lactose-Binding Lectin 4, L-36 Lactose-Binding Protein, L36LBP, Lectin,Galactoside-Binding Soluble 4
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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