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Bovine Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit

SKU:
BODL00051
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
F1N3T9
Sensitivity:
6.5pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
B-FGF,BFGF,FGFB,HBGH-2,Basic Fibroblast Growth Factor,Heparin-binding growth factor 2
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Bovine Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit

The Bovine Fibroblast Growth Factor 2 (bFGF2) ELISA Kit is specifically designed for the accurate measurement of bFGF2 levels in bovine serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring precise and consistent results, making it an invaluable tool for a variety of research purposes.bFGF2 is a key growth factor that plays a vital role in promoting cell proliferation, migration, and differentiation. It is essential for tissue repair, wound healing, and embryonic development.

Dysregulation of bFGF2 levels has been implicated in various diseases, including cancer, cardiovascular disorders, and neurological conditions, making it a critical biomarker for understanding these diseases and exploring potential therapeutic interventions.The Bovine Fibroblast Growth Factor 2 ELISA Kit from Assay Genie provides researchers with a reliable and efficient method for quantifying bFGF2 levels, facilitating deeper insights into the role of this important growth factor in both health and disease.

Product Name:Bovine Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit
Product Code:BODL00051
Size:96 Assays
Target:FGF2
Synonyms:B-FGF, BFGF, FGFB, HBGH-2, Basic Fibroblast Growth Factor, Heparin-binding growth factor 2
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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