The Bovine Defensin Beta-1 (DEFB1) ELISA Kit is a powerful tool for detecting and quantifying levels of DEFB1 in bovine serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers precise and dependable results, making it well-suited for a variety of research applications.DEFB1 is a key component of the innate immune system, known for its antimicrobial properties and ability to combat pathogens. Studies have shown that DEFB1 is involved in various biological processes, including inflammation, wound healing, and immune response modulation.
By measuring DEFB1 levels, researchers can gain valuable insights into bovine health, host-pathogen interactions, and immune system function.Whether investigating infectious diseases, studying host defense mechanisms, or exploring immune system regulation, the DEFB1 ELISA Kit offers researchers a reliable tool for advancing their understanding of bovine biology. Trust in its accuracy and precision to drive your research forward.
Product Name:
Bovine Defensin Beta 1 (DEFb1) ELISA Kit
Product Code:
BODL00043
Size:
96 Assays
Target:
DEFb1
Synonyms:
B-DF1, DEFB1, BD1, DEF-B1, DEFB101, HBD1
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
