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Bovine Caspase 3 (CASP3) ELISA Kit

SKU:
BODL00027
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q08DY9
Sensitivity:
0.071ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CPP32,CPP32B,SCA1,Apoptain,Yama,Apoptosis-Related Cysteine Peptidase,Cysteinyl Aspartate Specific Proteinases 3,SREBP cleavage activity 1
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Bovine Caspase 3 (CASP3) ELISA Kit

The Bovine Caspase-3 (CASP3) ELISA Kit is specifically designed for the precise quantification of caspase-3 levels in bovine serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it a valuable tool for various research applications.Caspase-3 is a key enzyme in the process of apoptosis, playing a crucial role in cell death and survival pathways. Dysregulation of caspase-3 activity has been implicated in a variety of diseases, including cancer, autoimmune disorders, and neurodegenerative conditions.

By measuring caspase-3 levels, researchers can gain insights into disease mechanisms and potential therapeutic targets.The Bovine Caspase-3 ELISA Kit from AssayGenie provides a reliable and efficient method for studying caspase-3 biology in bovine samples, facilitating advancements in biomedical research and drug development.

Product Name:Bovine Caspase 3 (CASP3) ELISA Kit
Product Code:BODL00027
Size:96 Assays
Target:CASP3
Synonyms:CPP32, CPP32B, SCA1, Apoptain, Yama, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 3, SREBP cleavage activity 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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