Bovine Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit
The Bovine Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit is a powerful tool for the quantification of BMPR1A levels in bovine samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and reliable results, making it ideal for a variety of research applications.BMPR1A is a key receptor involved in bone morphogenesis and development, playing a crucial role in regulating cell growth and differentiation. Dysregulation of BMPR1A signaling has been implicated in various diseases, including skeletal disorders and cancer, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
By accurately measuring BMPR1A levels, researchers can gain insights into the molecular mechanisms underlying these diseases and identify novel targets for drug development. Trust the Bovine BMPR1A ELISA Kit for accurate and reproducible results, advancing your research in the field of bone biology and beyond.
Product Name:
Bovine Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit
Product Code:
BODL00026
Size:
96 Assays
Target:
BMPR1A
Synonyms:
CD292, ACVRLK3, ALK3, SKR5, BMPR1-A, Activin A Receptor, Type Ii-Like Kinase 3, Activin Receptor-Like Kinase 3, Serine/Threonine-Protein Kinase Receptor R5
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
