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Bovine B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

SKU:
BODL00021
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O02718
Sensitivity:
0.66ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
B-Cell CLL/Lymphoma 2
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Bovine B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

The Bovine B-Cell Leukemia/Lymphoma 2 (BCL2) ELISA Kit is a powerful tool for quantifying BCL2 levels in bovine samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an ideal choice for various research applications.BCL2 is a key protein involved in regulating cell survival and apoptosis, making it a crucial factor in the development and progression of leukemia and lymphoma in cattle.

By accurately measuring BCL2 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new treatment options.Whether studying bovine health, investigating disease progression, or evaluating therapeutic interventions, the BCL2 ELISA Kit offers a reliable and efficient solution for researchers looking to advance their understanding of B-cell leukemia and lymphoma in cattle.

Product Name:Bovine B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit
Product Code:BODL00021
Size:96 Assays
Target:Bcl2
Synonyms:B-Cell CLL/Lymphoma 2
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)83-9890
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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