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Bovine Aspartate Aminotransferase (AST) ELISA Kit

SKU:
BODL00019
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P33097
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
cCAT,AAT,ASAT,SGOT,GOT1,Cysteine transaminase, cytoplasmic,Transaminase A,Aspartate Transaminase 1,Cytoplasmic,Glutamic-Oxaloacetic Transaminase 1,Soluble
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$899
Frequently bought together:

Description

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Bovine Aspartate Aminotransferase (AST) ELISA Kit

The Bovine Aspartate Aminotransferase (AST) ELISA Kit from AssayGenie is a high-quality assay designed for the accurate detection of AST levels in bovine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an excellent choice for researchers in various fields.Aspartate Aminotransferase is an important enzyme that plays a key role in amino acid metabolism. Elevated levels of AST in the blood can indicate damage to the liver or heart, making it a valuable biomarker for studying liver and heart diseases in bovines.

This ELISA kit provides researchers with a powerful tool for studying these conditions and developing potential therapies.Overall, the Bovine Aspartate Aminotransferase (AST) ELISA Kit from AssayGenie is a valuable resource for researchers looking to accurately measure AST levels in bovine samples and gain insights into liver and heart health in bovines.

Product Name:Bovine Aspartate Aminotransferase (AST) ELISA Kit
Product Code:BODL00019
Size:96 Assays
Target:AST
Synonyms:cCAT, AAT, ASAT, SGOT, GOT1, Cysteine transaminase, cytoplasmic, Transaminase A, Aspartate Transaminase 1,Cytoplasmic, Glutamic-Oxaloacetic Transaminase 1,Soluble
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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