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Bovine Aprotinin (AP) ELISA Kit

SKU:
BODL00016
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P00974
Sensitivity:
0.43ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Competitive
Synonyms:
BPTI,Trasylol,Pancreatic Trypsin Inhibitor,Basic protease inhibitor
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Bovine Aprotinin (AP) ELISA Kit

The Bovine Aprotinin AP ELISA Kit is a reliable and accurate tool for detecting levels of aprotinin in bovine serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring consistent and reproducible results for a variety of research applications.Aprotinin is a protease inhibitor that plays a crucial role in regulating blood clotting and preventing excessive bleeding.

It is commonly used in laboratory research and pharmaceutical applications for its anti-inflammatory and anti-fibrinolytic properties. The Bovine Aprotinin AP ELISA Kit is essential for studying the functions of aprotinin in various biological processes and developing potential therapies for conditions related to blood clotting disorders.

Product Name:Bovine Aprotinin (AP) ELISA Kit
Product Code:BODL00016
Size:96 Assays
Target:AP
Synonyms:BPTI, Trasylol, Pancreatic Trypsin Inhibitor, Basic protease inhibitor
Detection Method:Competition
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)82-10292
EDTA plasma (n=5)90-10095
Heparin plasma (n=5)88-10094
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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