The Bovine Alpha-Lactalbumin (ALA) ELISA Kit is specifically designed for the accurate quantification of alpha-lactalbumin levels in bovine serum, plasma, and milk samples. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.Alpha-lactalbumin is a key whey protein known for its various biological functions, including antimicrobial properties and immune-enhancing effects. It is an essential component in infant formula and plays a crucial role in promoting infant growth and development.
Studying alpha-lactalbumin levels can provide valuable insights into lactation biology, dairy processing, and infant nutrition research.Our Bovine Alpha-Lactalbumin ELISA Kit is a reliable tool for researchers in the dairy industry, nutrition science, and animal health fields. With its user-friendly protocol and high-quality components, this kit is designed to meet the demands of accurate alpha-lactalbumin quantification, allowing for in-depth analysis and understanding of bovine milk protein dynamics.
Product Name:
Bovine Alpha-Lactalbumin (aLA) ELISA Kit
Product Code:
BODL00008
Size:
96 Assays
Target:
aLA
Synonyms:
LALBA LYZL7 Lactose Synthase B Protein Lysozyme Like Protein 7
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
