The BOLA2 Polyclonal Antibody (PACO60344) is a vital tool for researchers studying the BOLA2 protein, which is involved in iron metabolism and cellular function. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in various applications, including Western blotting. By binding to the BOLA2 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for investigations in the fields of biochemistry and molecular biology.BOLA2 is a crucial player in iron-sulfur cluster biogenesis and transfer, essential processes for cell survival and function.
Dysregulation of BOLA2 has been linked to disorders such as anemia and neurodegenerative diseases, highlighting its importance in maintaining cellular health. Research on BOLA2 may provide insights into novel therapeutic approaches for conditions related to iron metabolism and mitochondrial function. In conclusion, the BOLA2 Polyclonal Antibody is a valuable tool for researchers aiming to elucidate the role of BOLA2 in cellular processes and disease pathogenesis. Its specificity and reliability make it a cornerstone for studies in iron biology, redox homeostasis, and mitochondrial function.
IHC image of PACO60344 diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO60344 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO60344 diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins. Acts together with the monothiol glutaredoxin GLRX3.
Synonyms:
BolA-like protein 2, BOLA2; BOLA2B, BOLA2A;
UniProt Protein Function:
BOLA2B: Belongs to the bolA/yrbA family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 16p11.2
UniProt Protein Details:
NCBI Summary:
This gene is located within a region of a segmental duplication on chromosome 16p. The product of this gene belongs to the eukaryotic subfamily of the BolA-like proteins. This gene encodes the BolA-like protein 2. The BolA-like proteins are widely conserved from prokaryotes to eukaryotes, and these proteins seem to be involved in cell proliferation or cell-cycle regulation. This duplicated region contains a complete copy of the short splice variant of the BOLA2 gene and a partial copy of the long splice variant of the BOLA2 gene. [provided by RefSeq, Jul 2008]
