The BNIP3 Polyclonal Antibody (CAB5683) is a valuable tool for researchers studying BNIP3, a key protein involved in apoptosis and mitophagy processes. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting BNIP3 in human samples, making it ideal for Western blot applications. By binding to the BNIP3 protein, this antibody enables precise detection and analysis of BNIP3 expression in various cell types, facilitating research in the fields of cell biology, cancer biology, and neurology.BNIP3, a member of the Bcl-2 family of proteins, plays a crucial role in regulating cell survival and death pathways by promoting mitochondrial dysfunction and autophagy.
Dysregulation of BNIP3 expression has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Therefore, investigating the functions and mechanisms of BNIP3 using the CAB5683 antibody can provide valuable insights into disease pathogenesis and potential therapeutic targets related to mitochondrial dysfunction and cellular homeostasis.
Product Name:
BNIP3 Rabbit Polyclonal Antibody
SKU:
CAB5683
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-120 of human BNIP3 (NP_004043.3).
This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation.
Purification Method:
Affinity purification
Gene ID:
664
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using BNIP3 Rabbit pAb (CAB5683) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
