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BNIP3 Rabbit Polyclonal Antibody (CAB5683)

SKU:
CAB5683
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
  • Cell Death Antibodies 2 Anti-BNIP3 Antibody CAB5683
  • Cell Death Antibodies 2 Anti-BNIP3 Antibody CAB5683
  • Cell Death Antibodies 2 Anti-BNIP3 Antibody CAB5683
  • Cell Death Antibodies 2 Anti-BNIP3 Antibody CAB5683
  • Cell Death Antibodies 2 Anti-BNIP3 Antibody CAB5683
$167 - $503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:BNIP3 Rabbit Polyclonal Antibody
SKU:CAB5683
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-120 of human BNIP3 (NP_004043.3).
Sequence:AFPP PAAE AHPA ARRG LRSP QLPS GAMS QNGA PGMQ EESL QGSW VELH FSNN GNGG SVPA SVSI YNGD MEKI LLDA QHES G
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:NIP3; HABON; BNIP3
Positive Sample:HeLa,NIH/3T3,Rat heart
Conjugate:Unconjugated
Cellular Localization:Mitochondrion, Mitochondrion outer membrane, Single-pass membrane protein.
Calculated MW:22kDa
Observed MW:22-70kDa

This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation.

Purification Method:Affinity purification
Gene ID:664
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using BNIP3 Rabbit pAb (CAB5683) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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