The BMI1 Polyclonal Antibody (CAB0211) is designed for research involving the BMI1 protein, a key regulator of stem cell self-renewal and proliferation. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it a valuable tool for studies in stem cell biology, cancer research, and developmental biology.BMI1 is a member of the Polycomb Group (PcG) proteins that are involved in epigenetic regulation and play a crucial role in maintaining the self-renewal capacity of stem cells.
Dysregulation of BMI1 has been implicated in various cancers, making it a potential therapeutic target for cancer treatments.This antibody has been validated for use in Western blot applications, enabling the detection and analysis of BMI1 protein levels in different cell types and tissues. By understanding the function and regulation of BMI1, researchers can gain insights into its role in stem cell maintenance, cancer progression, and potential therapeutic strategies.
Product Name:
Bmi1 Rabbit Polyclonal Antibody
SKU:
CAB0211
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Bmi1 (NP_005171.4).
Sequence:
LKDY YTLM DIAY IYTW RRNG PLPL KYRV RPTC KRMK ISHQ RDGL TNAG ELES DSGS DKAN SPAG GIPS TSSC LPSP STPV QSPH PQFP HISS TMNG TSNS P
Tested Applications:
WBIPELISA
Recommended Dilution:
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene.
Purification Method:
Affinity purification
Gene ID:
648
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Bmi1 Rabbit pAb (CAB0211) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
