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Blood Ammonia Assay Kit - Colorimetric (MAES0116)

SKU:
MAES0116
Product Type:
Assay
Detection Method:
Colorimetric
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma
Sensitivity:
0.01 mmol/L
Range:
0.01-2.5 mmol/L
Assay Time:
45 min
Shipping:
Gel Pack
Research Area:
Ferroptosis
Kidney Biomarker
Liver Biomarker
Amino Acid and Protein
€389
Frequently bought together:

Description

system_update_altDatasheet
Product Name:Blood Ammonia Assay Kit - Colorimetric
Product Code:MAES0116
Product Size:96 Assays
Sample Type:Serum, plasma
Assay Time:45 min
Instrument:Microplate reader
Sensitivity:0.01 mmol/L
Detection Range:0.01-2.5 mmol/L
Recovery Rate:103
Inter CV:7.2
Inter CV:4.1

Ammonia (NH3) or its ionic form is an important source of nitrogen in biological systems. Ammonia is a metabolite produced by the deamination of amino acids. In living systems, glutamic acid dehydrogenase and glutamine synthetase are key regulators of amino acid and ammonia metabolism. Glutamic acid dehydrogenase catalyzes the oxidation of glutamic acid to α-ketoglutarate and ammonia, and glutamic acid Aminamide synthase is used to eliminate excess ammonia.

Blood protein can be precipitated with protein precipitator, and enzyme activity will be destroyed, which can prevent the formation of free ammonia in vitro. Most interfering color substances were removed at the same time, indigo was formed in non-protein filtrate by Berthelot reaction, and the color depth was proportional to the content of blood ammonia. Blood ammonia content can be determined by comparing with standard solution.

Shelf Life:3 Months
Storage:2-8°C
Shipping:Gel Pack
Research Area:Liver Biomarkers, Amino acids & proteins

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