The BLM Polyclonal Antibody (PACO05015) is a valuable tool for researchers studying the BLM protein, a key player in DNA replication and repair processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the BLM protein, researchers can detect and analyze its expression in various cell types, making it an essential component in studies related to genetics, cancer biology, and aging research.BLM, also known as Bloom syndrome protein, is crucial for maintaining genomic stability and preventing the accumulation of DNA damage. Mutations in the BLM gene have been associated with increased cancer susceptibility and premature aging syndromes.
Research focused on understanding the function of BLM can provide insights into mechanisms of DNA repair and potentially lead to the development of targeted cancer therapies and strategies for addressing age-related disorders.Overall, the BLM Polyclonal Antibody (PACO05015) serves as a valuable tool for investigating the role of BLM in various biological processes and diseases, offering researchers the opportunity to delve deeper into the complexities of genomic maintenance and its implications for human health.
Antibody Name:
BLM Antibody (PACO05015)
Antibody SKU:
PACO05015
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC, IF
Recommended Dilutions:
ELISA:1:5000, IHC:1:100-1:300, IF:1:200-1:1000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from human BLM around the non-phosphorylation site of T99.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
BLM; RECQ2; RECQL3; Bloom syndrome protein; DNA helicase; RecQ-like type 2; RecQ2; RecQ protein-like 3
UniProt Protein Function:
BLM: a magnesium-dependent ATP-dependent DNA-helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. A member of the RecQ helicase family that is required for genome stability. Participates in DNA replication, recombination and repair. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex which is a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2.Protein type: DNA repair, damage; Helicase; DNA replication; EC 3.6.4.12Chromosomal Location of Human Ortholog: 15q26.1Cellular Component: chromosome, telomeric region; cytoplasm; lateral element; nuclear chromosome; nuclear matrix; nucleolus; nucleoplasm; nucleus; PML bodyMolecular Function: ATP binding; ATP-dependent 3'-5' DNA helicase activity; ATP-dependent DNA helicase activity; ATP-dependent helicase activity; ATPase activity; bubble DNA binding; four-way junction helicase activity; G-quadruplex DNA binding; helicase activity; p53 binding; protein binding; single-stranded DNA bindingBiological Process: DNA double-strand break processing; DNA replication; DNA synthesis during DNA repair; double-strand break repair via homologous recombination; mitotic cell cycle G2/M transition DNA damage checkpoint; negative regulation of cell division; negative regulation of DNA recombination; positive regulation of transcription, DNA-dependent; protein oligomerization; protein sumoylation; regulation of cyclin-dependent protein kinase activity; replication fork processing; response to DNA damage stimulus; response to X-ray; strand displacementDisease: Bloom Syndrome
UniProt Protein Details:
NCBI Summary:
The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008]
