The Bleomycin Hydrolase Polyclonal Antibody (CAB6535) is an essential tool for researchers studying bleomycin hydrolase, an enzyme involved in the inactivation of the anti-cancer drug bleomycin. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it suitable for Western blot applications.Bleomycin hydrolase plays a crucial role in the metabolism and detoxification of bleomycin, a chemotherapy agent commonly used to treat various types of cancer. By targeting this enzyme, researchers can gain insights into drug resistance mechanisms and develop novel strategies to enhance the efficacy of bleomycin-based therapies.
This polyclonal antibody enables precise detection and quantification of bleomycin hydrolase in cell lysates and tissue samples, facilitating in-depth investigations into its functions and potential therapeutic implications. Its versatility and reliability make it an invaluable tool for studies in oncology and drug development, with the potential to improve treatment outcomes for cancer patients.
Product Name:
BLMH Rabbit Polyclonal Antibody
SKU:
CAB6535
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BLMH (NP_000377.1).
Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily.
Purification Method:
Affinity purification
Gene ID:
642
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using BLMH Antibody (CAB6535) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
