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BL-CAM (Phospho-Tyr807) Colorimetric Cell-Based ELISA Kit

SKU:
CBCAB01664
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Research Area:
Cell Biology
Reactivity:
Human
Detection Method:
Colorimetric
€599
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Description

system_update_altDatasheetMSDS

BL-CAM (Phospho-Tyr807)Colorimetric Cell-Based ELISA Kit

The BL-CAM Phospho-Tyr807 Colorimetric Cell-based ELISA Kit is a cutting-edge assay designed for the accurate detection of phosphorylated tyrosine 807 on the BL-CAM (Basal Cell Adhesion Molecule) protein in cell lysates and tissue extracts. This kit offers high sensitivity and specificity, ensuring precise and reliable results for research applications in cell signaling pathways and protein phosphorylation.BL-CAM is a cell adhesion molecule that plays a crucial role in various cellular processes, including cell adhesion, migration, and signaling. Phosphorylation of tyrosine 807 on BL-CAM is known to regulate its function and signaling pathways, making it a valuable target for studying cell-cell interactions and intracellular signaling cascades.

Whether investigating cancer cell metastasis, immune cell activation, or developmental processes, the BL-CAM Phospho-Tyr807 Colorimetric Cell-based ELISA Kit provides researchers with a powerful tool to study the role of phosphorylated BL-CAM in cellular functions and disease mechanisms. With its ease of use and robust performance, this kit is essential for researchers striving to deepen their understanding of cell biology and signaling pathways.

Product Name:BL-CAM (Phospho-Tyr807) Colorimetric Cell-Based ELISA
Product Code:CBCAB01664
ELISA Type:Cell-Based
Target:BL-CAM (Phospho-Tyr807)
Reactivity:Human
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nm
Format:2 x 96-Well Microplates

The BL-CAM (Phospho-Tyr807) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect BL-CAM protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated BL-CAM in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on BL-CAM phosphorylation.

Qualitative determination of BL-CAM (Phospho-Tyr807) concentration is achieved by an indirect ELISA format. In essence, BL-CAM (Phospho-Tyr807) is captured by BL-CAM (Phospho-Tyr807)-specific primary (1ø) antibodies while the HRP-conjugated secondary (2ø) antibodies bind the Fc region of the 1ø antibody. Through this binding, the HRP enzyme conjugated to the 2ø antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 933, UniProt ID: P20273, OMIM: 107266, Unigene: Hs.709215
Gene Symbol:CD22
Sub Type:Phospho
UniProt Protein Function:CD22: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: integral to plasma membrane; external side of plasma membrane

Molecular Function:protein binding; carbohydrate binding

Biological Process: cell adhesion

UniProt Code:P20273
NCBI GenInfo Identifier:6166019
NCBI Gene ID:933
NCBI Accession:P20273.2
UniProt Secondary Accession:P20273,O95699, O95701, O95702, O95703, Q01665, Q32M46 Q92872, Q92873, Q9UQA6, F5GYU4, F5H7U3,
UniProt Related Accession:P20273
Molecular Weight:75,354 Da
NCBI Full Name:B-cell receptor CD22
NCBI Synonym Full Names:CD22 molecule
NCBI Official Symbol:CD22  
NCBI Official Synonym Symbols:SIGLEC2; SIGLEC-2  
NCBI Protein Information:B-cell receptor CD22; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2
UniProt Protein Name:B-cell receptor CD22
UniProt Synonym Protein Names:B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD_antigen: CD22
Protein Family:CD226 antigen
UniProt Gene Name:CD22  
UniProt Entry Name:CD22_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37 °C prior to adding cells.
2.Incubate the cells for overnight at 37 °C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4 °C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies Anti-BL-CAM (Phospho-Tyr807) Antibody, Anti-BL-CAM Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4 °C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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