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Biotinylated Recombinant Human Glypican-3/GPC3 Protein (RPCB0050)

SKU:
RPCB0050
Size:
100µg
Tag:
C-His&Avi
Reactivity:
Human
€1,079

Description

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Product Name:Biotinylated Recombinant Human Glypican-3/GPC3 Protein
SKU:RPCB0050
Size:100µg
Tag:C-His&Avi
Reactivity:Human
Protein Description:High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human Glypican-3/GPC3 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:< 1 EU/μg of the protein by LAL method.
Purity:> 95% by Tris-Bis PAGE
Formulation:Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Gene ID:2719

Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.

Storage:Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:P51654
Product ImageBiotinylated Human GPC3 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.
Product ImageUse Biotinylated Human GPC3-His protein to detect the expression rate of Anti-GPC3-CAR positive cells. 293T cells and anti-GPC3 CAR-293T cells were incubated with Biotinylated human GPC3-His (25-559) , His Tag and Biotinylated Protein Control-His. Non-transfected 293 cells and Biotinylated Protein Control-His were used as negative control. SA-FITC was used to evaluate the binding activity of Biotinylated Human GPC3.

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