Biotinylated Recombinant Human B7-2/CD86 Protein (RPCB0674)
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- SKU:
- RPCB0674
- Size:
- 100µg
- Tag:
- C-His&Avi
- Reactivity:
- Human
Description
| Product Name: | Biotinylated Recombinant Human B7-2/CD86 Protein |
| SKU: | RPCB0674 |
| Size: | 100µg |
| Tag: | C-His&Avi |
| Reactivity: | Human |
| Protein Description: | High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human B7-2/CD86 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method |
| Purity: | > 95% by SDS-PAGE |
| Formulation: | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Gene ID: | 942 |
B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P42081-1 |
 | Biotinylated Human B7-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
 | Immobilized Biotinylated Human B7-2, His Tag at 1 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.9ng/mL determined by ELISA. |
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