The Biotinylated Anti-CD47 Antibody (HDLA014) is a valuable tool for researchers studying CD47, a cell surface protein known for its role in immune evasion and cancer progression. This antibody, produced and validated for use in various applications, including immunohistochemistry and flow cytometry, specifically binds to the CD47 protein, allowing for precise detection and analysis in different types of cells.CD47, also known as integrin-associated protein (IAP), is a key player in immune regulation, acting as a "don't eat me" signal that helps cancer cells evade the immune system. Its overexpression has been linked to poor prognosis in various cancer types, making it an attractive target for therapeutic interventions.
This antibody provides researchers with a reliable tool to investigate CD47 expression and function, facilitating studies in immunology, oncology, and drug development.By using the Biotinyalted Anti-CD47 Antibody (HDLA014), researchers can gain insights into the mechanisms by which CD47 promotes immune evasion and tumor growth, paving the way for the development of novel targeted therapies aimed at disrupting this pathway. Its reliability and versatility make it an essential reagent for any laboratory focused on advancing our understanding of cancer biology and immunotherapy.
SKU:
HDLA014
Size:
100 µg
Clonality:
Monoclonal
Clone:
DM49
Synonyms:
CD47, MER6, IAP, OA3
Applications:
ELISA, Flow Cyt
Recommended Dilution:
ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:
Rabbit
Isotype:
Rabbit IgG
Reactivity:
Human
Purification Method:
Purified from cell culture supernatant by affinity chromatography
Formulation:
Powder
Buffer:
1XPBS
Storage:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized antibodies are shipped at ambient temperature.
Usage:
Research use only
Background:
Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
