The Biotinylated Anti-CD33 Antibody (HDLA039) offered by Assay Genie is a valuable tool for researchers studying CD33, a cell surface protein expressed on myeloid cells and involved in immune responses. This antibody, developed for use in various applications including immunofluorescence and flow cytometry, is highly specific to human CD33 samples.CD33 is a key target for therapies in hematological malignancies, making this antibody essential for investigating the role of CD33 in diseases like acute myeloid leukemia (AML) and myelodysplastic syndromes.
By utilizing this antibody, researchers can further explore the mechanisms of CD33 in immune regulation and potential therapeutic strategies for targeting CD33-positive cells.With its high specificity and reliability, the Biotinylated Anti-CD33 Antibody (HDLA039) from Assay Genie is a valuable tool for advancing research in oncology and immunology, providing crucial insights into the functions of CD33 and its implications in disease pathology.
Product Code:
HDLA039
Size:
100 µg
Clonality:
Monoclonal
Clone:
DM77
Synonyms:
CD33,SIGLEC3,gp67
Applications:
ELISA, Flow Cyt
Recommended Dilution:
ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:
Rabbit
Isotype:
Rabbit IgG
Reactivity:
Human
Purification Method:
Purified from cell culture supernatant by affinity chromatography
Formulation:
Powder
Buffer:
1XPBS
Storage:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized antibodies are shipped at ambient temperature.
Usage:
Research use only
Background:
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K.
