The Biotinidase Polyclonal Antibody (CAB6284) is a valuable tool for research involving the enzyme biotinidase, which plays a crucial role in biotin metabolism. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting.Biotinidase is responsible for recycling biotin, a B-vitamin essential for various metabolic pathways, and deficiency in this enzyme can lead to severe neurological and developmental issues. By targeting biotinidase, researchers can gain insights into biotin metabolism and potential therapeutic strategies for disorders related to biotin deficiency.
With its specificity and reliability, the Biotinidase Polyclonal Antibody (CAB6284) enables accurate detection and analysis of biotinidase in different sample types, making it an ideal choice for studies focused on biotin metabolism, enzymology, and related diseases. Don't miss the opportunity to advance your research with this high-quality antibody.
Product Name:
BTD Rabbit Polyclonal Antibody
SKU:
CAB6284
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 284-543 of human BTD (NP_000051.1).
The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
686
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using BTD Rabbit pAb (CAB6284) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
