The Biotin Monoclonal Antibody (CAB22315) is a specialized tool for researchers studying the protein biotin, known for its essential role in cell metabolism and DNA synthesis. This antibody, produced through monoclonal antibody technology, offers high specificity and sensitivity for detecting biotin in various biological samples, particularly in immunohistochemistry and ELISA applications. By targeting the biotin molecule, researchers can accurately visualize and quantify biotin levels in cells and tissues, providing valuable insights into biotin-related processes and functions.
Biotin, a water-soluble B vitamin, is crucial for maintaining healthy skin, hair, and nails, as well as supporting energy production and gene expression. Research on biotin deficiency, supplementation, and bioavailability requires reliable tools like the Biotin Monoclonal Antibody (CAB22315) to effectively study biotin's impact on cellular pathways and physiological functions. With its high affinity and specificity, this antibody offers a reliable option for investigating biotin-related research questions in diverse fields such as nutrition, metabolism, and biomedicine.
Product Name:
Biotin Monoclonal Antibody
SKU:
CAB22315
Size:
20uL, 100uL
Isotype:
IgG1
Host Species:
Rabbit
Reactivity:
Other (Wide Range Predicted)
Immunogen:
Biotin
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Conjugate:
Unconjugated
Observed MW:
Refer to figures
Biotin is an organic heterobicyclic compound that consists of 2-oxohexahydro-1H-thieno[3,4-d]imidazole having a valeric acid substituent attached to the tetrahydrothiophene ring. The parent of the class of biotins. It has a role as a prosthetic group, a coenzyme, a nutraceutical, a human metabolite, a Saccharomyces cerevisiae metabolite, an Escherichia coli metabolite, a mouse metabolite, a cofactor and a fundamental metabolite. It is a member of biotins and a vitamin B7. It is a conjugate acid of a biotinate.
Purification Method:
Affinity purification
Gene ID:
CAS:58-85-5
Clone Number:
ARC50706
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Biotin Rabbit mAb (CAB22315) at1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
