The Bin2 Antibody (PACO56736) is a high-quality polyclonal antibody designed for research involving Bin2, a protein associated with regulating immune responses and cellular processes. This antibody, produced and purified in a controlled environment, is highly specific and reacts strongly with human samples. Validated for use in Western blot applications, the Bin2 Antibody enables researchers to detect and analyze Bin2 protein expression in various cell types.Bin2, also known as Bridging integrator 2, plays a critical role in cell signaling and immune response modulation. Its involvement in cellular processes and immune regulation makes it a valuable target for studies in immunology, cancer research, and inflammation-related diseases.
Understanding the functions and interactions of Bin2 can provide insights into the development of targeted therapies for conditions such as cancer, autoimmune disorders, and inflammatory diseases.The Bin2 Antibody offers researchers a reliable tool for investigating the role of Bin2 in cellular pathways and disease processes. Its high specificity and sensitivity make it an essential component for research projects aiming to uncover the mechanisms underlying immune regulation and disease progression.
Immunofluorescence staining of HepG2 cells with PACO56736 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO56736 diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO56736 diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Promotes cell motility and migration, probably via its interaction with the cell membrane and with podosome proteins that mediate interaction with the cytoskeleton. Modulates membrane curvature and mediates membrane tubulation. Plays a role in podosome formation. Inhibits phagocytosis.
Synonyms:
Bridging integrator 2 (Breast cancer-associated protein 1), BIN2, BRAP1
UniProt Protein Function:
Promotes cell motility and migration, probably via its interaction with the cell membrane and with podosome proteins that mediate interaction with the cytoskeleton. Modulates membrane curvature and mediates membrane tubulation. Plays a role in podosome formation. Inhibits phagocytosis.
