The BID Polyclonal Antibody (CAB0210) is specifically designed for research involving the BID protein, a key player in the regulation of apoptosis (cell death) and mitochondrial function. This antibody is raised in rabbits and has been validated for use in Western blot applications, demonstrating high reactivity with human samples. By binding to the BID protein, this antibody enables researchers to detect and analyze BID expression in a variety of cell types, making it an ideal tool for studies in cell biology, cancer research, and apoptosis regulation.BID, a member of the Bcl-2 protein family, functions as a critical mediator in the apoptotic pathway, linking extrinsic death receptor signaling to the intrinsic mitochondrial pathway.
Dysregulation of BID has been implicated in various diseases, including cancer, neurodegenerative disorders, and immune-related conditions. By studying the role of BID in these contexts, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.Overall, the BID Polyclonal Antibody (CAB0210) offers researchers a reliable tool for investigating the functions of the BID protein in various cellular processes and disease states. Its high specificity and sensitivity make it a valuable asset for advancing our understanding of apoptosis regulation and developing targeted therapies for a range of medical conditions.
Product Name:
Bid Rabbit Polyclonal Antibody
SKU:
CAB0210
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human Bid (NP_001187.1).
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found.
Purification Method:
Affinity purification
Gene ID:
637
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Bid Rabbit pAb (CAB0210) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
