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beta -hydroxybutyryl-HIST1H2BC (K20) Antibody (PACO60512)

SKU:
PACO60512
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
ICC
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • beta -hydroxybutyryl-HIST1H2BC K20 Antibody PACO60512
  • beta -hydroxybutyryl-HIST1H2BC K20 Antibody PACO60512
$419
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Description

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beta -hydroxybutyryl-HIST1H2BC (K20) Antibody (PACO60512)

The Beta-Hydroxybutyryl-Hist1H2Bc (K20) Antibody (PAC060512) is a valuable tool for researchers studying the histone modification beta-hydroxybutyrylation. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in various research applications, including Western blotting.Histone modifications play a crucial role in regulating gene expression and chromatin structure, and beta-hydroxybutyrylation is a recently discovered modification that has implications for cellular metabolism and gene regulation. The Beta-Hydroxybutyryl-Hist1H2Bc (K20) Antibody specifically targets the beta-hydroxybutyrylation of histone H2B at lysine 20, enabling researchers to investigate the role of this modification in various cellular processes.

This antibody is ideal for studies in epigenetics, metabolism, and gene regulation, providing researchers with a valuable tool for exploring the functional significance of beta-hydroxybutyrylation in health and disease. Its high specificity and reactivity make it a reliable choice for researchers seeking to deepen their understanding of this emerging histone modification pathway.

Antibody Name:?-hydroxybutyryl-HIST1H2BC (K20) Antibody (PACO60512)
Antibody SKU:PACO60512
Size:50ul
Host Species:Rabbit
Tested Applications:ELISA, WB, ICC
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:100-1:1000, ICC:1:20-1:200
Species Reactivity:Human
Immunogen:Peptide sequence around site of β -hydroxybutyryl-Lys (20) derived from Human Histone H2B type 1-C/E/F/G/I
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:Antigen Affinity Purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern Blot. Detected samples: 293 whole cell lysate, A549 whole cell lysate; Untreated (-) or treated (+) with 50mM Sodium 3-hydroxybutyrate for 72h. All lanes: HIST1H2BC antibody at 1:100. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 14 kDa. Observed band size: 14 kDa.
Product ImageImmunocytochemistry analysis of PACO60512 diluted at 1:40 and staining in Hela cells (treated with 50mM sodium 3-hydroxybutyrate for 72h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms:Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK
UniProt Protein Function:H2B1C: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: cytoplasm; extracellular space; nucleoplasm; nucleus

Molecular Function:DNA binding; protein binding

Biological Process: antibacterial humoral response; defense response to Gram-positive bacterium; innate immune response in mucosa; nucleosome assembly

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:P62807
NCBI GenInfo Identifier:290457686
NCBI Gene ID:8339
NCBI Accession:P62807.4
UniProt Secondary Accession:P62807,P02278, Q3B872, Q4VB69, Q93078, Q93080,
UniProt Related Accession:P62807
Molecular Weight:13,906 Da
NCBI Full Name:Histone H2B type 1-C/E/F/G/I
NCBI Synonym Full Names:histone cluster 1, H2bg
NCBI Official Symbol:HIST1H2BG  
NCBI Official Synonym Symbols:H2B/a; H2BFA; H2B.1A; dJ221C16.8  
NCBI Protein Information:histone H2B type 1-C/E/F/G/I
UniProt Protein Name:Histone H2B type 1-C/E/F/G/I
UniProt Synonym Protein Names:Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l
Protein Family:Histone
UniProt Gene Name:HIST1H2BC  
UniProt Entry Name:H2B1C_HUMAN
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