The Beta-Galactosidase Polyclonal Antibody (CAB1863) is a valuable tool for researchers studying beta-galactosidase, an enzyme involved in lactose metabolism and widely used as a reporter gene in molecular biology techniques. This antibody, generated in rabbits, exhibits high specificity and sensitivity when detecting beta-galactosidase in various sample types, including human samples. It has been validated for use in Western blot applications, allowing for precise and accurate detection of the enzyme.Beta-galactosidase is a versatile enzyme with applications in various fields, including molecular biology, biochemistry, and biotechnology. Its role in lactose digestion and utilization makes it a key target for research on gastrointestinal health and lactose intolerance.
Additionally, its use as a reporter gene in gene expression studies and recombinant protein production highlights its importance in biotechnological applications.By utilizing the Beta-Galactosidase Polyclonal Antibody, researchers can effectively study the expression and activity of beta-galactosidase in different experimental contexts, providing valuable insights into its functions and potential applications. Whether investigating lactose metabolism, gene expression analysis, or protein purification, this antibody offers reliable and accurate detection of beta-galactosidase, empowering researchers in their scientific endeavors.
Product Name:
GLB1 Rabbit Polyclonal Antibody
SKU:
CAB1863
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 329-677 of human GLB1 (NP_000395.3).
This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome.
Purification Method:
Affinity purification
Gene ID:
2720
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using GLB1 Rabbit pAb (CAB1863) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
