null

Beta-agonist (Beta-Agonist) ELISA Kit (FSES0050)

SKU:
FSES0050
Product Type:
ELISA Kit
Applications:
ELISA
Research Area:
Food Science
$539
Frequently bought together:

Description

Technical Manual

Beta-agonist (Beta-Agonist) ELISA Kit

Kit Name: Beta-agonist (Beta-Agonist) ELISA Kit
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can detect Beta-agonist in samples, such as muscle, urine, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the detection, Beta-agonist in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti- Beta-agonist antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of Beta-agonist. The concentration of Beta-agonist in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 0.2 ppb (ng/mL)
Reaction Mode: 25°C,15 min~15 min~15 min.
Detection Limit: Urine, Muscle, Serum --- 1 ppb
Cross Reactivity:

Clenbuterol

100%

Clorprenaline,Clenpenterol

141%

Tulobuterol

80%

Brombuterol, Mapenterol

107%

Cimbuterol

86.9%

Salbutamol

60.6%

Mabuterol

56.3%

Terbutaline

33.1%

Bambuterol

18%

Cimaterol

14%

Fenoterol,Clenbuterol-hydroxymethyl

10.6%

Clenproperol

5%

Ractopamine, Zilpaterol, Phenylethanolamine A

<1%
Sample Recovery Rate:
Kits Components:
Item Specifications
ELISA Microtiter Plate 96 wells
Standard Liquid 1 mL each<       (0 ppb, 0.2 ppb, 0.6 ppb, 1.8 ppb, 5.4 ppb, 16.2 ppb)
HRP Conjugate 12 mL
Antibody Working Solution 7 mL
Substrate Reagent A 6 mL
Substrate Reagent B 6 mL
Stop Solution 6 mL
Sample Stabilizer 5 mL
Sample Diluent 30 mL
20×Concentrated Wash Buffer 25 mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy
Instruments: Microplate reader, Homogenizer, Oscillators, Centrifuge, Balance (sensibility 0.01 g).
Micropipette: Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents: Trichloroacetic acid, NaOH.
Storage And Expiry Date: Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.

Related Products