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Bcl-2 Polyclonal Antibody (CAB2845)

SKU:
CAB2845
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
IF
IP
€129 - €359
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Bcl-2 Polyclonal Antibody
SKU:CAB2845
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-239 of human Bcl-2 (NP_000624.2).
Sequence:MAHA GRTG YDNR EIVM KYIH YKLS QRGY EWDA GDVG AAPP GAAP APGI FSSQ PGHT PHPA ASRD PVAR TSPL QTPA APGA AAGP ALSP VPPV VHLT LRQA GDDF SRRY RRDF AEMS SQLH LTPF TARG RFAT VVEE LFRD GVNW GRIV AFFE FGGV MCVE SVNR EMSP LVDN IALW MTEY LNRH LHTW IQDN GGWD AFVE LYGP SMRP LFDF SWLS LKTL LSLA LVGA CITL GAYL GHK
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:Bcl-2; PPP1R50
Positive Sample:Jurkat,Mouse lung
Conjugate:Unconjugated
Cellular Localization:Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein.
Calculated MW:26kDa
Observed MW:26kDa

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:596
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Jurkat cells, using Bcl-2 Rabbit pAb (CAB2845) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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