The Bcl-2 Polyclonal Antibody (CAB2845) is a vital tool for researchers studying the B-cell lymphoma-2 (Bcl-2) protein, a key regulator of cell survival and apoptosis. The antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the Bcl-2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential tool for studies in cancer biology and immunology.Bcl-2 is a critical player in apoptosis regulation, with dysregulation of its expression linked to various cancers and other diseases.
By investigating the function and regulation of Bcl-2, researchers can gain insights into the mechanisms underlying cell survival and death pathways, offering potential targets for therapeutic intervention in cancer and other related conditions. The Bcl-2 Polyclonal Antibody is a valuable asset for research aimed at unraveling the complexities of cell apoptosis and survival mechanisms.
Product Name:
Bcl-2 Polyclonal Antibody
SKU:
CAB2845
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-239 of human Bcl-2 (NP_000624.2).
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
596
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Bcl-2 Rabbit pAb (CAB2845) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.