The BCL-2 Polyclonal Antibody (CAB16775) is a vital tool for researchers studying the BCL-2 protein, a key regulator of apoptosis (programmed cell death). This antibody, generated in rabbits, exhibits high specificity for human samples and has been validated for use in Western blotting applications. By binding to the BCL-2 protein, this antibody enables the accurate detection and analysis of BCL-2 expression in a variety of cell types, making it an essential component of studies in cell biology and cancer research.BCL-2 is a critical player in the regulation of cell survival and apoptosis, with dysregulation of its expression often associated with various diseases, including cancer.
By targeting BCL-2, researchers can gain insights into the mechanisms of cell death and survival, potentially uncovering new therapeutic strategies for treating cancer and other disorders characterized by aberrant cell proliferation and survival. The BCL-2 Polyclonal Antibody is a valuable tool for advancing our understanding of these processes and developing novel treatment approaches.
Product Name:
Bcl-2 Polyclonal Antibody
SKU:
CAB16775
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bcl-2 (NP_000624.2).
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
596
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse lung, using Bcl-2 Rabbit pAb (CAB16775) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 5s.
