The BCKDK Polyclonal Antibody (CAB8184) is a valuable tool for researchers studying the BCKDK protein, which plays a crucial role in branched-chain amino acid metabolism. This antibody, generated in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to the BCKDK protein, this antibody allows for the detection and analysis of BCKDK in a variety of cell types, making it an essential component of studies in metabolism and metabolic disorders.BCKDK, also known as branched-chain ketoacid dehydrogenase kinase, is involved in regulating the activity of the branched-chain ketoacid dehydrogenase complex, which is responsible for breaking down branched-chain amino acids.
Dysregulation of BCKDK has been linked to various metabolic disorders, making it a potential target for therapeutic interventions in conditions such as diabetes, obesity, and metabolic syndrome. Research on the function and regulation of BCKDK is crucial for advancing our understanding of metabolic pathways and developing targeted treatments for metabolic disorders.
Product Name:
BCKDK Rabbit Polyclonal Antibody
SKU:
CAB8184
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 153-412 of human BCKDK (NP_005872.2).
The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10295
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of 22Rv1 cells, using BCKDK antibody (CAB8184) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
