The BCKDHB Polyclonal Antibody (CAB10533) is a valuable tool for researchers studying the BCKDHB protein, which is involved in the branched-chain alpha-keto acid dehydrogenase complex. This complex plays a crucial role in the breakdown of branched-chain amino acids, which are essential for protein synthesis and energy production in the body.Raised in rabbits, this antibody is highly specific and reactive with human samples, making it suitable for use in Western blot applications. By binding to the BCKDHB protein, researchers can detect and analyze its expression in various cell types, providing insights into its functions and potential implications in metabolic disorders and related diseases.
The BCKDHB Polyclonal Antibody is a valuable tool for studies in biochemistry, metabolism, and genetics, offering a deeper understanding of the molecular mechanisms underlying metabolic processes and the potential development of targeted therapies. Its versatility and reliability make it an essential component of research efforts aimed at unraveling the complexities of metabolic pathways and their roles in health and disease.
Product Name:
BCKDHB Rabbit Polyclonal Antibody
SKU:
CAB10533
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 183-392 of human BCKDHB (NP_898871.1).
This gene encodes the E1 beta subunit of branched-chain keto acid dehydrogenase, which is a multienzyme complex associated with the inner membrane of mitochondria. This enzyme complex functions in the catabolism of branched-chain amino acids. Mutations in this gene have been associated with maple syrup urine disease (MSUD), type 1B, a disease characterized by a maple syrup odor to the urine in addition to mental and physical retardation and feeding problems. Alternative splicing at this locus results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
594
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using BCKDHB antibody (CAB10533) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
