The BCKDHB Monoclonal Antibody (CAB23916) is a valuable tool for researchers studying the BCKDHB protein, a key enzyme involved in branched-chain amino acid metabolism. This antibody, produced using monoclonal technology, shows high specificity and sensitivity for detecting BCKDHB in various biological samples.BCKDHB is essential for breaking down branched-chain amino acids, which play crucial roles in protein synthesis and energy production. Dysregulation of BCKDHB activity has been linked to various metabolic disorders, making it a target of interest in metabolic research and potential therapeutic interventions.
The CAB23916 antibody is validated for use in techniques like Western blotting and immunohistochemistry, allowing for precise detection and localization of BCKDHB in cell and tissue samples. Its high specificity and reliability make it a valuable tool for studying the role of BCKDHB in metabolic pathways and exploring its potential implications in disease mechanisms.
Product Name:
BCKDHB Monoclonal Antibody
SKU:
CAB23916
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 51-392 of human BCKDHB (NP_898871.1).
This gene encodes the E1 beta subunit of branched-chain keto acid dehydrogenase, which is a multienzyme complex associated with the inner membrane of mitochondria. This enzyme complex functions in the catabolism of branched-chain amino acids. Mutations in this gene have been associated with maple syrup urine disease (MSUD), type 1B, a disease characterized by a maple syrup odor to the urine in addition to mental and physical retardation and feeding problems. Alternative splicing at this locus results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
594
Clone Number:
ARC62321
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using BCKDHB Rabbit mAb (CAB23916) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
