The BCKDHA Polyclonal Antibody (CAB16774) is a valuable tool for researchers studying the BCKDHA protein, which is a key enzyme involved in branched-chain amino acid metabolism. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.BCKDHA plays a crucial role in the breakdown of branched-chain amino acids, such as leucine, isoleucine, and valine, serving as a critical component of the branched-chain alpha-keto acid dehydrogenase complex. Dysregulation of BCKDHA activity has been implicated in various metabolic disorders, making it an important target for research in metabolic pathways and diseases.
By specifically binding to the BCKDHA protein, this antibody enables accurate detection and analysis of BCKDHA expression in different cell types, facilitating studies in metabolism, nutrition, and related fields. Understanding the role of BCKDHA is essential for unraveling the complexities of amino acid metabolism and developing targeted therapies for metabolic disorders.
Product Name:
BCKDHA Rabbit Polyclonal Antibody
SKU:
CAB16774
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 276-445 of human BCKDHA (NP_000700.1).
The branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). This gene encodes the alpha subunit of the decarboxylase (E1) component. Mutations in this gene result in maple syrup urine disease, type IA. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
593
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using BCKDHA Rabbit pAb (CAB16774) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
