The BBOX1 Antibody (CAB5810) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. RRID AB_2766562 Gene ID 8424 Swiss Prot Synonym BBH; BBOX; G-BBH; gamma-BBH; BBOX1
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
BBOX1 Antibody
SKU:
CAB5810
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:100 - 1:500
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BBH, BBOX, G-BBH, gamma-BBH, BBOX1
Positive Sample:
Mouse testis, Rat liver
Cellular Localization:
Cytoplasm.
Calculated MW:
45kDa
Observed MW:
45kDa
This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. RRID AB_2766562 Gene ID 8424 Swiss Prot Synonym BBH; BBOX; G-BBH; gamma-BBH; BBOX1
Purification Method:
Affinity purification
Gene ID:
8424
RRID:
AB_2766562
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using BBOX1 Rabbit pAb (CAB5810) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse kidney using BBOX1 Rabbit pAb (CAB5810) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
