The BBC3 Polyclonal Antibody (CAB17138) is a valuable tool for researchers studying the BBC3 gene, which is known to play a key role in apoptosis, or programmed cell death. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications.The BBC3 protein, also known as PUMA (p53 upregulated modulator of apoptosis), is involved in the regulation of cell death pathways and is a critical mediator of p53-induced apoptosis. Its role in promoting cell death in response to cellular stress makes it an important target for understanding the mechanisms of cancer development and therapy resistance.
By specifically binding to the BBC3 protein, this antibody enables the detection and analysis of BBC3 expression in various cell types, providing valuable insights into its function in apoptosis regulation. Researchers in the fields of oncology, cell biology, and molecular biology will find this antibody essential for their studies on cancer biology and therapeutic strategies targeting apoptosis pathways.
Product Name:
PUMA Rabbit Polyclonal Antibody
SKU:
CAB17138
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-131 of human PUMA (NP_001120713.1).
This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
27113
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PUMA Rabbit pAb (CAB17138) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
